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Alternative names, keywords |
Vitamin B12, cobalamin (click here for folate) |
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Samples required |
● Clotted blood (gold cap, 5 mL tube). Smaller tubes are available for paediatric samples. Vitamin B12 determinations should be performed on samples from fasting patients. |
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Test indications |
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Factors affecting test performance/ results |
The assay is designed to avoid interference due to anti-intrinsic factor antibodies. Biotin may interfere - samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration. Samples with extremely high total protein concentrations are not suitable for use in this assay. The critical total protein concentration is dependent upon the individual sample composition.. The presence of immunoglobulin-vitamin B12 complexes may cause unexpectedly high values of vitamin B12. In rare cases, interference due to extremely high titres of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. Results should always be assessed in conjunction with the patient’s medical history, clinical examination and other findings. |
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Reference range |
Adults: 197 - 771 ng/L Source: Roche Cobas® Vitamin B12 II kit insert, 2023-11, V 9.0 Paediatric: <1 month: 187 - 1866 ng/L Source: https://caliperdatabase.org/ for Roche Cobas vitamin B12, except for 1-11y lower limit (in-house data) and 12-18y lower limit (as adult Roche range). |
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Turnaround time |
24 hours (weekdays) |
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Enquiries |
Biochemistry (Automation) |